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FERGUS-GREENE
KATHLEEN FERGUS
1915 23RD AVENUE
SAN FRANCISCO, CA, 94116
US
Clones are generated with enhanced gene trap vectors. 8247 new gene trap sequence tags released. Release of the new GGTC web interface. More than 35000 gene trap sequence tags analyzed by genomic PCR.
Several collections of mutated mouse embryonic stem cell lines are available. The objective of the joint IRBM-TIGEM TRAP-FLIP project is to demonstrate the feasibility of constructing a library of ES cell clones in which each expressed gene is tagged with cassette exchange sites. Our system is based on a novel types of gene trap vectors containing two heterospecific FRT sites, and on exchange vectors designed to exchange the trapped cassette with any gene of interest.